Pancreatic islets, and in particular insulin producing beta-cells, can be monitored under different conditions of nutrient supply. The metabolic measurement of beta-cells or islets can provide basic information on their viability and functional capacity in regenerative medicine.
Here, changes in the metabolic activity of the beta-cell line, INS-1E, are detected when exposed to glucose concentrations equivalent to physiologically low and high blood glucose levels; conditions which regulate insulin secretion.
Cellular model: INS-1E pancreatic beta cells as 2D monolayer
To investigate e.g. anti-inflammatory properties of food additives interactions between cells and additive can be monitored.
The example shows the interference between an additive and escherichia coli bacteria after 9 h of interaction.
To investigate the potential of algae for biofuel production the vitality of the algae can be monitored during changing environmental conditions.
Algae undergoing photo-synthetic activity under luminous conditions produce oxygen and consume more oxygen when under darkened conditions.
Cellular model: Local Japanese algae as suspension